Item no. |
PRS-25-131-100ul |
Manufacturer |
ProSci
|
Amount |
100 ul |
Category |
|
Type |
Antibody Polyclonal |
Format |
Liquid |
Applications |
WB, ELISA |
Specific against |
Human, Mouse |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
SP3, DKFZp686O1631, SPR-2, SPR2 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Transcription , Cancer |
Immunogen |
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SP3. |
Applications |
SP3 antibody can be used for detection of SP3 by ELISA at 1:312500. SP3 antibody can be used for detection of SP3 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50, 000 - 100, 000. |
Positive Control 1 |
Cat. No. XBL-10413 - Fetal Skeletal Muscle Tissue Lysate |
Predicted Molecular Weight |
82 kDa |
Purification |
Antibody is purified by peptide affinity chromatography method. |
Clonality |
Polyclonal |
Conjugate |
Unconjugated |
Buffer |
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Concentration |
batch dependent |
Storage Conditions |
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store SP3 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles. |
Disclaimer |
This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
SP3 |
Accession # |
NP_003102 |
Protein Gi # |
38373693 |
Ncbi Gene Id # |
6670 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
SP3 |
Ncbi Official Full Name |
Sp3 transcription factor |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q02447 |
Background |
SP3 is a transcriptional factor that can act as an activator or repressor, probably in a isoform-specific manner. SP3 binds to GT and GC boxes promoters elements.This gene belongs to a family of Sp1 related genes that encode transcription factors that regulate transcription by binding to consensus GC- and GT-box regulatory elements in target genes. This protein contains a zinc finger DNA-binding domain and several transactivation domains, and has been reported to function as a bifunctional transcription factor that either stimulates or represses the transcription of numerous genes. Transcript variants encoding different isoforms have been described for this gene, and one has been reported to initiate translation from a non-AUG (AUA) start codon. Additional isoforms, resulting from the use of alternate downstream translation initiation sites, have also been noted. |
Background References 1 |
Whitten, C., Gene 413 (1-2), 58-66 (2008). |
1st Image Caption |
Antibody used in IHC on Human Prostate at 5.0 ug/ml. |
2nd Image Caption |
Antibody used in WB on Human Muscle at 0.2-1 ug/ml. |
3rd Image Caption |
Antibody used in WB on Human U2OS at 1:1000. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.