CIKS Antibody

Signal Transduction

CIKS antibody can be used for detection of CIKS by Western blot at 1 μ g/mL. A band at approximately 63 kDa can be detected. Antibody can also be used for immunohistochemistry starting at 5 μ g/mL. For immunofluorescence start at 20 μ g/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Cat. No. 1302 - Human Lung Tissue Lysate

63 kDa

Polyclonal

Unconjugated

1 mg/ml

Cat. No. 2499P – CIKS Peptide

None

AF274303

10758

TRAF3IP2

Homo sapiens

CIKS Antibody: Nuclear factor kappa B (NF-κ B) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κ B mediates the expression of a great variety of genes in response to extracellular stimuli. NF-κ B associates with Iκ B proteins in the cell cytoplasm, which inhibit NF-κ B activity. Iκ B is phosphorylated by Iκ B kinase (IKK) complex that contains IKKα , IKKβ , and IKKγ . A novel molecule that associates with and activates IKK was recently identified and designated CIKS (for connection to IKK and SAPK/JNK) and Act1 (for NF-κ B activator 1). CIKS directly interacts with IKKγ . CIKS/Act1 also activates activating transcription factor (ATF) and activator protein 1 (AP-1) through Jun kinase (JNK). These results indicate that CIKS/Act1 is involved in the inflammation and stress responses. CIKS/Act1 is ubiquitously expressed in human tissues.

Li X, Commane M, Nie H, Hua X, Chatterjee-Kishore M, Wald D, Haag M, Stark GR. Act1, an NF-κ B activating protein. Proc Natl Acad Sci USA. 2000; 97(19):10489-93.

Immunohistochemistry of CIKS in human lung tissue with CIKS antibody at 5 μ g/mL.