Comparison

ATP5D Antibody

Item no. PRS-23-979-50uL
Manufacturer ProSci
Amount 50 uL
Category
Type Antibody Primary
Format Liquid
Applications WB
Specific against Human, Mouse, Rat
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias ATP synthase subunit delta, mitochondrial, F-ATPase delta subunit, ATP5D
Available
Shipping
blue ice or RT
By Research Area
Cancer, Neuroscience, Signal Transduction
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-168 of human ATP5D (NP_001678.1).
Applications
WB: 1:500 - 1:2000
Positive Control 1
MCF7
Positive Control 2
Raji
Positive Control 3
HepG2
Positive Control 4
A-549
Positive Control 5
Mouse kidney
Positive Control 6
Mouse heart
Predicted Molecular Weight
Observed: 17kDa
Purification
Affinity purification
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions
Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications
None
Ncbi Official Symbol
ATP5D
Ncbi Gene Id #
513
User Note
Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol
ATP5D
Ncbi Official Full Name
ATP synthase subunit delta, mitochondrial
Ncbi Organism
Homo sapiens
Swissprot #
P30049
Background
This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the delta subunit of the catalytic core. Alternatively spliced transcript variants encoding the same isoform have been identified.
1st Image Caption
Western blot analysis of extracts of various cell lines, using ATP5D antibody (23-979) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 50 uL
Available: In stock
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