Comparison

HIC1 Antibody

Item no. PRS-23-890-50uL
Manufacturer ProSci
Amount 50 uL
Category
Type Antibody Primary
Format Liquid
Applications WB, IHC
Specific against Human, Rat
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias HIon Channel1, hic-1, ZBTB29, ZNF901
Available
Shipping
blue ice or RT
By Research Area
Cancer
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 180-430 of human HIC1 (NP_001091672.1).
Applications
WB: 1:500 - 1:2000

IHC: 1:100 - 1:200
Positive Control 1
NCI-H460
Positive Control 2
HT-29
Positive Control 3
DU145
Positive Control 4
Rat heart
Predicted Molecular Weight
Observed: 76kDa
Purification
Affinity purification
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions
Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications
None
Ncbi Official Symbol
HIC1
Ncbi Gene Id #
3090
User Note
Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol
HIC1
Ncbi Official Full Name
hypermethylated in cancer 1
Ncbi Organism
Homo sapiens
Swissprot #
Q14526
Background
This gene functions as a growth regulatory and tumor repressor gene. Hypermethylation or deletion of the region of this gene have been associated with tumors and the contiguous-gene syndrome, Miller-Dieker syndrome. Alternative splicing of this gene results in multiple transcript variants.
1st Image Caption
Western blot analysis of extracts of various cell lines, using HIC1 antibody (23-890) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 50 uL
Available: In stock
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