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YWHAH Antibody

YWHAH Antibody

Item no.	PRS-23-787-50uL
Manufacturer	ProSci
Amount	50 uL
Category	Antibodies Immunohistochemistry Western Blot Immunofluorescence Primary Antibodies
Type	Antibody Primary
Format	Liquid
Applications	WB, IF, IHC
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	YWHAH, YWHA1
Available
Shipping	blue ice or RT
By Research Area	Apoptosis, Cell Cycle, Signal Transduction, Stem Cell
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-246 of human YWHAH (NP_003396.1).
Applications	WB: 1:500 - 1:2000 IHC: 1:50 - 1:100 IF: 1:50 - 1:100
Positive Control 1	U-87MG
Positive Control 2	LO2
Positive Control 3	Raji
Positive Control 4	Jurkat
Positive Control 5	Mouse heart
Positive Control 6	Mouse kidney
Predicted Molecular Weight	Observed: 28kDa
Purification	Affinity purification
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions	Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	YWHAH
Ncbi Gene Id #	7533
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	YWHAH
Ncbi Official Full Name	tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta
Ncbi Organism	Homo sapiens
Swissprot #	Q04917
Background	This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and bovine orthologs. This gene contains a 7 bp repeat sequence in its 5' UTR, and changes in the number of this repeat have been associated with early-onset schizophrenia and psychotic bipolar disorder.
1st Image Caption	Western blot analysis of extracts of various cell lines, using YWHAH antibody (23-787) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
2nd Image Caption	Immunofluorescence analysis of NIH-3T3 cells using YWHAH Polyclonal Antibody (23-787) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
3rd Image Caption	Immunofluorescence analysis of NIH-3T3 cells using YWHAH Polyclonal Antibody (23-787) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 50 uL

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Delivery expected until 10/4/2024

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