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MKL1 Antibody

MKL1 Antibody

Item no.	PRS-23-628-50uL
Manufacturer	ProSci
Amount	50 uL
Category	Oncology Antibodies By Cell Type Hematology Leukemia Immunohistochemistry Immunoprecipitation Western Blot Immunofluorescence Primary Antibodies By Organ Heart & Blood Cancer
Type	Antibody Primary
Format	Liquid
Applications	WB, IF, IP, IHC
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	MKL/myocardin-like protein 1, Megakaryoblastic leukemia 1 protein, Megakaryocytic acute leukemia protein, Myocardin-related transcription factor A, MRTF-A, MKL1, KIAA1438, MAL
Available
Shipping	blue ice or RT
By Research Area	Apoptosis, Cell Cycle, Signal Transduction, Stem Cell, Transcription
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human MKL1 (NP_065882.1).
Applications	WB: 1:500 - 1:2000 IHC: 1:50 - 1:200 IF: 1:50 - 1:200 IP: 1:20 - 1:50
Positive Control 1	TE-1
Positive Control 2	HeLa
Positive Control 3	Mouse lung
Positive Control 4	Rat testis
Predicted Molecular Weight	Observed: 145kDa
Purification	Affinity purification
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions	Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	MKL1
Ncbi Gene Id #	57591
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	MKL1
Ncbi Official Full Name	MKL/myocardin-like protein 1
Ncbi Organism	Homo sapiens
Swissprot #	Q969V6
Background	The protein encoded by this gene interacts with the transcription factor myocardin, a key regulator of smooth muscle cell differentiation. The encoded protein is predominantly nuclear and may help transduce signals from the cytoskeleton to the nucleus. This gene is involved in a specific translocation event that creates a fusion of this gene and the RNA-binding motif protein-15 gene. This translocation has been associated with acute megakaryocytic leukemia. Alternative splicing results in multiple transcript variants.
1st Image Caption	Western blot analysis of extracts of various cell lines, using MKL1 antibody (23-628) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
2nd Image Caption	Immunofluorescence analysis of U-2 OS cells using MKL1 Polyclonal Antibody (23-628) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 50 uL

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Delivery expected until 9/6/2024