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TUBB8 Antibody

TUBB8 Antibody

Item no.	PRS-23-553-50uL
Manufacturer	ProSci
Amount	50 uL
Category	Antibodies Cell Biology Cytoskeleton Immunohistochemistry Western Blot Immunofluorescence Primary Antibodies Mikrotubules
Type	Antibody Primary
Format	Liquid
Applications	WB, IF, IHC
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Tubulin beta-8 chain, TUBB8
Available
Shipping	blue ice or RT
By Research Area	Cell Cycle, Signal Transduction
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 140-444 of human TUBB8 (NP_817124.1).
Applications	WB: 1:500 - 1:2000 IHC: 1:50 - 1:100 IF: 1:50 - 1:200
Positive Control 1	U-251MG
Positive Control 2	Jurkat
Positive Control 3	K-562
Positive Control 4	Mouse brain
Predicted Molecular Weight	Observed: 50kDa
Purification	Affinity purification
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions	Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	TUBB8
Ncbi Gene Id #	347688
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	TUBB8
Ncbi Official Full Name	Tubulin beta-8 chain
Ncbi Organism	Homo sapiens
Swissprot #	Q3ZCM7
Background	The protein encoded by this gene represents the primary beta-tubulin subunit of oocytes and the early embryo. Defects in this gene, which is primate-specific, are a cause of oocyte maturation defect 2 and infertility.
1st Image Caption	Western blot analysis of extracts of various cell lines, using TUBB8 antibody (23-553) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
2nd Image Caption	Immunohistochemistry of paraffin-embedded rat lung using TUBB8 antibody (23-553) at dilution of 1:100 (40x lens).
3rd Image Caption	Immunohistochemistry of paraffin-embedded mouse stomach using TUBB8 antibody (23-553) at dilution of 1:100 (40x lens).
4th Image Caption	Immunofluorescence analysis of U2OS cells using TUBB8 antibody (23-553) at dilution of 1:100. Blue: DAPI for nuclear staining.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 50 uL

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Delivery expected until 9/6/2024