Item no. |
PRS-23-526-50uL |
Manufacturer |
ProSci
|
Amount |
50 uL |
Category |
|
Type |
Antibody Primary |
Format |
Liquid |
Applications |
WB, IHC |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
RPN2, RIBIIR, RPN-II, RPNII, SWP1 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Other |
Immunogen |
Recombinant fusion protein containing a sequence corresponding to amino acids 360-540 of human RPN2 (NP_002942.2). |
Applications |
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100 |
Positive Control 1 |
HepG2 |
Positive Control 2 |
293T |
Positive Control 3 |
Jurkat |
Positive Control 4 |
MCF7 |
Positive Control 5 |
NIH/3T3 |
Positive Control 6 |
Mouse liver |
Predicted Molecular Weight |
Observed: 69kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
RPN2 |
Ncbi Gene Id # |
6185 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
RPN2 |
Ncbi Official Full Name |
ribophorin II |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P04844 |
Background |
This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein is similar in sequence to the yeast oligosaccharyl transferase subunit SWP1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
1st Image Caption |
Western blot analysis of extracts of various cell lines, using RPN2 antibody (23-526) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s. |
2nd Image Caption |
Immunohistochemistry of paraffin-embedded rat brain using RPN2 antibody (23-526) at dilution of 1:100 (40x lens). |
3rd Image Caption |
Immunohistochemistry of paraffin-embedded human stomach using RPN2 antibody (23-526) at dilution of 1:100 (40x lens). |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.