Comparison

BSND Antibody

Item no. PRS-23-152-50uL
Manufacturer ProSci
Amount 50 uL
Category
Type Antibody Primary
Format Liquid
Applications WB, IHC
Specific against Human, Mouse, Rat
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias Barttin, BSND, BART
Available
Shipping
blue ice or RT
By Research Area
Cancer, Neuroscience, Signal Transduction
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 54-320 of human BSND (NP_476517.1).
Applications
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Positive Control 1
293T
Positive Control 2
Rat liver
Positive Control 3
Rat kidney
Positive Control 4
Rat intestine
Predicted Molecular Weight
Observed: 35kDa
Purification
Affinity purification
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions
Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications
None
Ncbi Official Symbol
BSND
Ncbi Gene Id #
7809
User Note
Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol
BSND
Ncbi Official Full Name
Barttin
Ncbi Organism
Homo sapiens
Swissprot #
Q8WZ55
Background
This gene encodes an essential beta subunit for CLC chloride channels. These heteromeric channels localize to basolateral membranes of renal tubules and of potassium-secreting epithelia of the inner ear. Mutations in this gene have been associated with Bartter syndrome with sensorineural deafness.
1st Image Caption
Western blot analysis of extracts of various cell lines, using BSND antibody (23-152) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 60s.
2nd Image Caption
Immunohistochemistry of paraffin-embedded rat kidney using BSND antibody (23-152) at dilution of 1:100 (40x lens).
3rd Image Caption
Immunohistochemistry of paraffin-embedded mouse kidney using BSND antibody (23-152) at dilution of 1:100 (40x lens).

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 50 uL
Available: In stock
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