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CAST Antibody, KO Validated

CAST Antibody, KO Validated

Item no.	PRS-23-067-50uL
Manufacturer	ProSci
Amount	50 uL
Category	Neuroscience Molecular Targets for Neuroscience Antibodies Cell Biology Cell Status Apoptosis Western Blot Primary Antibodies
Type	Antibody Primary
Format	Liquid
Applications	WB
Specific against	Human, Mouse
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Calpastatin, Calpain inhibitor, BS-17, Calpastatin
Available
Shipping	blue ice or RT
By Research Area	Cell Cycle, Neuroscience
Immunogen	A synthetic peptide corresponding to a sequence within amino acids 550-650 of human CAST (NP_001177371.1).
Applications	WB: 1:500 - 1:2000
Positive Control 1	A-549
Positive Control 2	SW480
Positive Control 3	MCF-7
Positive Control 4	HepG2
Positive Control 5	Mouse lung
Positive Control 6	Rat liver
Predicted Molecular Weight	Observed: 130kDa
Validation	Antibody is Knockout validated.
Purification	Affinity purification
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions	Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	CAST
Ncbi Gene Id #	831
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	CAST
Ncbi Official Full Name	calpastatin
Ncbi Organism	Homo sapiens
Swissprot #	P20810
Background	The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described.
1st Image Caption	Western blot analysis of extracts of various cell lines, using CAST antibody (23-067) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
2nd Image Caption	Western blot analysis of extracts from normal (control) and CAST knockout (KO) HeLa cells, using CAST antibody (23-067) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 50 uL

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Delivery expected until 9/6/2024

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