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RIP3 Antibody

RIP3 Antibody

Item no.	PRS-2283-0.02mg
Manufacturer	ProSci
Amount	0.02 mg
Quantity options	0.02 mg 0.1 mg
Category	Antibodies Immunohistochemistry Immunoprecipitation Western Blot ELISA Immunofluorescence Primary Antibodies
Type	Antibody Polyclonal
Format	Liquid
Applications	WB, IF, IP, ELISA, IHC-P
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Rabbit
Isotype	IgG
Conjugate/Tag	Unconjugated
Citations	He et al. Receptor interacting protein kinase-3 determines cellular necrotic response to TNF-alpha. Cell. 2009;137(6):1100-11. PMID: 19524512 Zhang et al. Functional complementation between FADD and RIP1 in embryos and lymphocytes. Nature. 2011;471(7338):373-6. PMID: 21368761 Narayan et al. The NAD-dependent deacetylase SIRT2 is required for programmed necrosis. Nature. 2012;492(7428):199-204. PMID: 23201684 Li et al. Tissue damage negatively regulates LPS-induced macrophage necroptosis. Cell Death Differ. 2016;23(9):1428-47. doi:10.1038/cdd.2016.21. Epub 2016. PMID: 26943325 Yang et al. Regulation of RIP3 by the transcription factor Sp1 and the epigenetic regulator UHRF1 modulates cancer cell necroptosis. Cell Death Dis. 2017;8(10):e3084. doi:10.1038/cddis.2017.483. PMID: 28981102 Ramachandran et al. Receptor interacting protein kinase 3 is a critical early mediator of acetaminophen-induced hepatocyte necrosis in mice. Hepatology. 2013;58(6):2099-108. PMID: 23744808 Li et al. The RIP1/RIP3 necrosome forms a functional amyloid signaling complex required for programmed necrosis. Cell. 2012 ;150(2):339-50. PMID: 22817896 Vitner et al. RIPK3 as a potential therapeutic target for Gaucher's disease. Nat Med. 2014;20(2):204-8. PMID: 24441827 Wang et al. RNA viruses promote activation of the NLRP3 inflammasome through a RIP1-RIP3-DRP1 signaling pathway. Nat Immunol. 2014;15(12):1126-33. PMID: 25326752 Onizawa et al. The ubiquitin-modifying enzyme A20 restricts ubiquitination of the kinase RIPK3 and protects cells from necroptosis. Nat Immunol. 2015;16(6):618-27. PMID: 25939025 Murphy et al. The pseudokinase MLKL mediates necroptosis via a molecular switch mechanism. Immunity. 2013;39(3):443-53. PMID: 24012422 Nogusa et al. RIPK3 Activates Parallel Pathways of MLKL-Driven Necroptosis and FADD-Mediated Apoptosis to Protect against Influenza A Virus. Cell Host Microbe 2016;20(1):13-24. PMID: 27321907 Pearson et al. EspL is a bacterial cysteine protease effector that cleaves RHIM proteins to block necroptosis and inflammation. Nat Microbiol. 2017 ;2:16258. PMID: 28085133 Yang et al. The end of RIPK1-RIPK3-MLKL-mediated necroptosis in acetaminophen-induced hepatotoxicity? Hepatology. 2016;64(1):311-2PMID: 26418225 Qu et al. Graphene oxide induces toll-like receptor 4 (TLR4)-dependent necrosisin macrophages. ACS Nano. 2013;7(7):5732-45.PMID: 23734789 Gandhirajan et al. Blockade of NOX2 and STIM1 signaling limits Lipo polysaccharide-induced vascular inflammation. J Clin Invest. 2013;123(2):887-902. PMID: 23348743 Fortes et al. Heme induces programmed necrosis on macrophages through autocrine TNF and ROS production. Blood. 2012;119(10):2368-75. PMID: 22262768 Xu et al. The cytoplasmic nuclear receptor RARγ controls RIP1 initiated cell death when cIAP activity is inhibited. Nat Commun. 2017;8(1):425. PMID: 28871172 Karunakaran et al. Targeting macrophage necroptosis for therapeutic and diagnostic interventions in atherosclerosis. Sci Adv. 2016 ;2(7):e1600224. PMID: 27532042 Chen et al. Mechanisms of necroptosis in T cells. J Exp Med. 2011 Apr 11;208(4):633-41.PMID: 21402742 Yu et al. Curr Biol. 1999;9(10):539-42. Sun et al. J Biol Chem. 1999;274(24):16871-5. Pazdernik et al. Mol Cell Bio. 1999; 19(10):6500-8 (WD0102)
NCBI	Ripk3
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	RIP3 Antibody: Rip3, AW107945, 2610528K09Rik, Rip3, RIP-like protein kinase 3, RIP-3
Available
Manufacturer - Applications	WB: 0.1-0.5 μg/mL; IHC-P: 5 μg/mL; IF: 20 μg/mL, IP: 20 μg/mL. Antibody validated: Western Blot in mouse and human samples; Immunohistochemistry in mouse and rat samples; Immunofluorescence in mouse and rat samples; Immunoprecipitation in human samples; Immunogold EM in mouse samples. All other applications and species not yet tested.
Shipping Temperature	blue ice or room temperature
Storage Conditions	RIP3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Molecular Weight	Predicted: 53kD for mouse RIP3 and 57kD for human RIP3 Observed: 53kD for mouse RIP3 and 57kD for human RIP3
Manufacturer - Research Area	Apoptosis
Background	RIP3 Antibody: Certain serine/threonine protein kinases, such as ASK1, RIP, DAP, and ZIP kinases, are mediators of apoptosis. Receptor interacting proteins including RIP and RIP2/RICK mediate apoptosis induced by TNFR1 and Fas, two prototype members in the death receptor family. A novel member in the RIP kinase family was recently identified and designated RIP3. RIP3 contains N-terminal kinase domain but, unlike RIP or RIP2, lacks the C-terminal death or CARD domain. RIP3 binds to RIP and TNFR1, mediates TNFR1 induced apoptosis, and attenuates RIP and TNFR1 induced NF-κ B activation. Overexpression of RIP3 induces apoptosis and NF-κ B activation. The messenger RNA of RIP3 is expressed in a subset of adult tissues.
Protein Gi #	6063101
Purification	RIP3 Antibody is affinity chromatography purified via peptide column.
Isoforms	Mouse RIP3 has one isoform(486aa, 53kD). Human RIP3 has 3 isoforms, including isoform 1 (518aa, 57kD), isoform 2 (252aa, 28kD) and isoform 3 (231aa, 25kD). Rat RIP3 also has one isoform (478aa, 52kD). 2283 can detect can detect isoforms of mouse and rat as well as human isoform 1.
Positive Control 2	PRS-1282-0.1mg
Positive Control 3	PRS-1285-0.1mg
Positive Control 4	PRS-1212-0.1mg
Concentration	1 mg/ml
Buffer	RIP3 Antibody is supplied in PBS containing 0.02% sodium azide.
Immunogen	Anti-RIP3 antibody (2283) was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of murine RIP3. The immunogen is located within the last 50 amino acids of RIP3.
Clonality	Polyclonal
NCBI Official Name	receptor-interacting serine-threonine kinase 3
NCBI Organism	Mus musculus
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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