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PPAN Antibody

PPAN Antibody

Item no.	PRS-22-900-50uL
Manufacturer	ProSci
Amount	50 uL
Category	Antibodies Immunohistochemistry Western Blot Immunofluorescence Primary Antibodies
Type	Antibody Primary
Format	Liquid
Applications	WB, IF, IHC
Specific against	Human, Mouse
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Suppressor of SWI4 1 homolog, Ssf-1, Brix domain-containing protein 3, Peter Pan homolog, PPAN, BXDC3, SSF1
Available
Shipping	blue ice or RT
By Research Area	Signal Transduction
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PPAN (NP_064615.3).
Applications	WB: 1:500 - 1:2000 IHC: 1:50 - 1:200 IF: 1:50 - 1:200
Positive Control 1	U-87MG
Positive Control 2	Mouse heart
Predicted Molecular Weight	Observed: 53kDa/55kDa
Purification	Affinity purification
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions	Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	PPAN
Ncbi Gene Id #	56342
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	PPAN
Ncbi Official Full Name	Suppressor of SWI4 1 homolog
Ncbi Organism	Homo sapiens
Swissprot #	Q9NQ55
Background	The protein encoded by this gene is an evolutionarily conserved protein similar to yeast SSF1 as well as to the gene product of the Drosophila gene peter pan (ppan). SSF1 is known to be involved in the second step of mRNA splicing. Both SSF1 and ppan are essential for cell growth and proliferation. Exogenous expression of this gene was reported to reduce the anchorage-independent growth of some tumor cells. Read-through transcription of this gene with P2RY11/P2Y(11), an adjacent downstream gene that encodes an ATP receptor, has been found. These read-through transcripts are ubiquitously present and up-regulated during granulocyte differentiation.
1st Image Caption	Western blot analysis of extracts of various cell lines, using PPAN Antibody (22-900) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
2nd Image Caption	Immunohistochemistry of paraffin-embedded human prostate using PPAN antibody (22-900) at dilution of 1:200 (40x lens).
3rd Image Caption	Immunohistochemistry of paraffin-embedded human stomach using PPAN antibody (22-900) at dilution of 1:200 (40x lens).
4th Image Caption	Immunofluorescence analysis of A549 cells using PPAN antibody (22-900).

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 50 uL

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Delivery expected until 10/4/2024

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