« Back
Home /
POLR2H Antibody

POLR2H Antibody

Item no.	PRS-22-578-50uL
Manufacturer	ProSci
Amount	50 uL
Category	Oncology Neuroscience Neural Lineage Markers Antibodies Immunoprecipitation Western Blot Primary Antibodies
Type	Antibody Primary
Format	Liquid
Applications	WB, IP
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	POLR2H, RPABC3, RPB17, RPB8, hsRPB8
Available
Shipping	blue ice or RT
By Research Area	Neuroscience
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human POLR2H (NP_006223.2).
Applications	WB: 1:500 - 1:2000 IP: 1:50 - 1:200
Positive Control 1	SW480
Positive Control 2	MCF-7
Positive Control 3	HL-60
Positive Control 4	293T
Positive Control 5	Mouse spleen
Positive Control 6	Mouse brain
Predicted Molecular Weight	Observed: 17kDa
Purification	Affinity purification
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions	Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	POLR2H
Ncbi Gene Id #	5437
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	POLR2H
Ncbi Official Full Name	polymerase (RNA) II (DNA directed) polypeptide H
Ncbi Organism	Homo sapiens
Swissprot #	P52434
Background	The three eukaryotic RNA polymerases are complex multisubunit enzymes that play a central role in the transcription of nuclear genes. This gene encodes an essential and highly conserved subunit of RNA polymerase II that is shared by the other two eukaryotic DNA-directed RNA polymerases, I and III. Alternative splicing results in multiple transcript variants of this gene.
1st Image Caption	Western blot analysis of extracts of various cell lines, using POLR2H antibody (22-578) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
2nd Image Caption	Immunoprecipitation analysis of 200ug extracts of MCF7 cells using 1ug POLR2H antibody (22-578). Western blot was performed from the immunoprecipitate using POLR2H antibody (22-578) at a dilition of 1:1000.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

Request product datasheet

Request safety datasheet

Simple Order Order Subscription

Amount: 50 uL

available

Delivery expected until 9/6/2024

Express Delivery: Save 21 days

Compare

Add to wishlist

Recommend by mail