Item no. |
PRS-22-497-50uL |
Manufacturer |
ProSci
|
Amount |
50 uL |
Category |
|
Type |
Antibody Primary |
Format |
Liquid |
Applications |
WB, IHC |
Specific against |
Human, Mouse |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
FANCL, POG, PHF9, PHD finger protein 9, FLJ10335, Fanconi anemia, complementation group L, FAAP43 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Cell Cycle |
Immunogen |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-255 of human FANCL (NP_060532.2). |
Applications |
WB: 1:200 - 1:2000
IHC: 1:20 - 1:200 |
Positive Control 1 |
Mouse thymus |
Predicted Molecular Weight |
Observed: 38kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
FANCL |
Ncbi Gene Id # |
55120 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
FANCL |
Ncbi Official Full Name |
Fanconi anemia, complementation group L |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q9NW38 |
Background |
The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group L. Alternative splicing results in two transcript variants encoding different isoforms. |
1st Image Caption |
Western blot analysis of extracts of mouse thymus, using FANCL antibody (22-497) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s. |
2nd Image Caption |
Immunohistochemistry of paraffin-embedded mouse stomach using FANCL antibody (22-497) at dilution of 1:100 (40x lens). |
3rd Image Caption |
Immunohistochemistry of paraffin-embedded human esophagus using FANCL antibody (22-497) at dilution of 1:100 (40x lens). |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.