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SURF1 Antibody

SURF1 Antibody

Item no.	PRS-22-466-50uL
Manufacturer	ProSci
Amount	50 uL
Category	Antibodies Immunohistochemistry Western Blot Primary Antibodies
Type	Antibody Primary
Format	Liquid
Applications	WB, IHC
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	CMT4K, surfeit locus protein 1, surfeit 1
Available
Shipping	blue ice or RT
By Research Area	Cancer, Signal Transduction
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 80-275 of human SURF1 (NP_003163.1).
Applications	WB: 1:500 - 1:2000 IHC: 1:50 - 1:200
Positive Control 1	SW480
Positive Control 2	HL-60
Positive Control 3	BT-474
Positive Control 4	SKOV3
Positive Control 5	U-251MG
Positive Control 6	Mouse liver
Predicted Molecular Weight	Observed: 33kDa
Purification	Affinity purification
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions	Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	SURF1
Ncbi Gene Id #	6834
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	SURF1
Ncbi Official Full Name	SURF1 cytochrome c oxidase assembly factor
Ncbi Organism	Homo sapiens
Swissprot #	Q15526
Background	This gene encodes a protein localized to the inner mitochondrial membrane and thought to be involved in the biogenesis of the cytochrome c oxidase complex. The protein is a member of the SURF1 family, which includes the related yeast protein SHY1 and rickettsial protein RP733. The gene is located in the surfeit gene cluster, a group of very tightly linked genes that do not share sequence similarity, where it shares a bidirectional promoter with SURF2 on the opposite strand. Defects in this gene are a cause of Leigh syndrome, a severe neurological disorder that is commonly associated with systemic cytochrome c oxidase deficiency.
1st Image Caption	Western blot analysis of extracts of various cell lines, using SURF1 antibody (22-466) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
2nd Image Caption	Immunohistochemistry of paraffin-embedded human colon carcinoma using SURF1 antibody (22-466) at dilution of 1:100 (40x lens).

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 50 uL

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Delivery expected until 9/6/2024

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