Item no. |
PRS-22-312-50uL |
Manufacturer |
ProSci
|
Amount |
50 uL |
Category |
|
Type |
Antibody Primary |
Format |
Liquid |
Applications |
WB, IF, IHC |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
CIRBP, CIRP |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Cell Cycle |
Immunogen |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-172 of human CIRBP (NP_001271.1). |
Applications |
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
IF: 1:10 - 1:100 |
Positive Control 1 |
MCF7 |
Positive Control 2 |
HL-60 |
Positive Control 3 |
BT-474 |
Positive Control 4 |
SKOV3 |
Positive Control 5 |
U-251MG |
Positive Control 6 |
Mouse brain |
Predicted Molecular Weight |
Observed: 18kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
CIRBP |
Ncbi Gene Id # |
1153 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
CIRBP |
Ncbi Official Full Name |
cold inducible RNA binding protein |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q14011 |
1st Image Caption |
Western blot analysis of extracts of various cell lines, using CIRBP antibody (22-312) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s. |
2nd Image Caption |
Immunohistochemistry of paraffin-embedded rat testis using CIRBP antibody (22-312) at dilution of 1:100 (40x lens). |
3rd Image Caption |
Immunohistochemistry of paraffin-embedded human breast cancer using CIRBP antibody (22-312) at dilution of 1:100 (40x lens). |
4th Image Caption |
Immunohistochemistry of paraffin-embedded mouse testis using CIRBP antibody (22-312) at dilution of 1:100 (20x lens). |
5th Image Caption |
Immunohistochemistry of paraffin-embedded mouse brain using CIRBP antibody (22-312) at dilution of 1:100 (40x lens). |
6th Image Caption |
Immunohistochemistry of paraffin-embedded mouse kidney using CIRBP antibody (22-312) at dilution of 1:100 (40x lens). |
7th Image Caption |
Immunofluorescence analysis of HeLa cells using CIRBP antibody (22-312). Blue: DAPI for nuclear staining. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.