Item no. |
PRS-22-295-50uL |
Manufacturer |
ProSci
|
Amount |
50 uL |
Category |
|
Type |
Antibody Primary |
Format |
Liquid |
Applications |
WB, IF, IHC |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Calumenin, Crocalbin, IEF SSP 9302, CALU |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Neuroscience, Signal Transduction |
Immunogen |
Recombinant fusion protein containing a sequence corresponding to amino acids 20-315 of human CALU (NP_001210.1). |
Applications |
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
IF: 1:50 - 1:200 |
Positive Control 1 |
NCI-H460 |
Positive Control 2 |
Mouse lung |
Predicted Molecular Weight |
Observed: 50kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
CALU |
Ncbi Gene Id # |
813 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
CALU |
Ncbi Official Full Name |
Calumenin |
Ncbi Organism |
Homo sapiens |
Swissprot # |
O43852 |
Background |
The product of this gene is a calcium-binding protein localized in the endoplasmic reticulum (ER) and it is involved in such ER functions as protein folding and sorting. This protein belongs to a family of multiple EF-hand proteins (CERC) that include reticulocalbin, ERC-55, and Cab45 and the product of this gene. Alternatively spliced transcript variants encoding different isoforms have been identified. |
1st Image Caption |
Western blot analysis of extracts of various cell lines, using CALU antibody (22-295) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit._Exposure time: 90s. |
2nd Image Caption |
Immunohistochemistry of paraffin-embedded mouse skin using CALU antibody (22-295) at dilution of 1:100 (40x lens). |
3rd Image Caption |
Immunohistochemistry of paraffin-embedded rat lung using CALU antibody (22-295) at dilution of 1:100 (40x lens). |
4th Image Caption |
Immunohistochemistry of paraffin-embedded mouse lung using CALU antibody (22-295) at dilution of 1:100 (40x lens). |
5th Image Caption |
Immunofluorescence analysis of NIH-3T3 cells using CALU antibody (22-295) at dilution of 1:100. Blue: DAPI for nuclear staining. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.