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HSP90B1 Antibody

HSP90B1 Antibody

Item no.	PRS-22-095-50uL
Manufacturer	ProSci
Amount	50 uL
Category	Oncology Neuroscience Aging & Neurological Disorders Antibodies By Cell Type Carcinoma Sarcoma Immunohistochemistry Western Blot Immunofluorescence Primary Antibodies
Type	Antibody Primary
Format	Liquid
Applications	WB, IF, IHC
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	HSP90B1, ECGP, GP96, TRA1, GRP94, HEL35, HEL-S-125m
Available
Shipping	blue ice or RT
By Research Area	Apoptosis, Cancer, Cell Cycle, Signal Transduction
Immunogen	A synthetic peptide corresponding to a sequence within amino acids 550-650 of human HSP90B1 (NP_003290.1).
Applications	WB: 1:500 - 1:2000 IHC: 1:50 - 1:200 IF: 1:50 - 1:200
Positive Control 1	HepG2
Positive Control 2	Jurkat
Positive Control 3	HeLa
Positive Control 4	NIH/3T3
Predicted Molecular Weight	Observed: 95kDa
Purification	Affinity purification
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions	Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	HSP90B1
Ncbi Gene Id #	7184
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	HSP90B1
Ncbi Official Full Name	heat shock protein 90kDa beta (Grp94), member 1
Ncbi Organism	Homo sapiens
Swissprot #	P14625
Background	This gene encodes a member of a family of adenosine triphosphate(ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation. There is a microRNA gene located within the 5' exon of this gene. There are pseudogenes for this gene on chromosomes 1 and 15.
1st Image Caption	Western blot analysis of extracts of various cell lines, using HSP90B1 antibody (22-095). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
2nd Image Caption	Immunofluorescence analysis of NIH-3T3 cells using HSP90B1 Polyclonal Antibody (22-095) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 50 uL

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Delivery expected until 9/6/2024