Item no. |
PRS-22-095-50uL |
Manufacturer |
ProSci
|
Amount |
50 uL |
Category |
|
Type |
Antibody Primary |
Format |
Liquid |
Applications |
WB, IF, IHC |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
HSP90B1, ECGP, GP96, TRA1, GRP94, HEL35, HEL-S-125m |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Apoptosis, Cancer, Cell Cycle, Signal Transduction |
Immunogen |
A synthetic peptide corresponding to a sequence within amino acids 550-650 of human HSP90B1 (NP_003290.1). |
Applications |
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200 |
Positive Control 1 |
HepG2 |
Positive Control 2 |
Jurkat |
Positive Control 3 |
HeLa |
Positive Control 4 |
NIH/3T3 |
Predicted Molecular Weight |
Observed: 95kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
HSP90B1 |
Ncbi Gene Id # |
7184 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
HSP90B1 |
Ncbi Official Full Name |
heat shock protein 90kDa beta (Grp94), member 1 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P14625 |
Background |
This gene encodes a member of a family of adenosine triphosphate(ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation. There is a microRNA gene located within the 5' exon of this gene. There are pseudogenes for this gene on chromosomes 1 and 15. |
1st Image Caption |
Western blot analysis of extracts of various cell lines, using HSP90B1 antibody (22-095). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
2nd Image Caption |
Immunofluorescence analysis of NIH-3T3 cells using HSP90B1 Polyclonal Antibody (22-095) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.