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APP Antibody

APP Antibody

Item no.	PRS-2136-0.1mg
Manufacturer	ProSci
Amount	0.1 mg
Category	Oncology Neuroscience Molecular Targets for Neuroscience Aging & Neurological Disorders Protein Aggregation, Clearance & Misfolding Antibodies By Cell Type Adenocarcinoma Immunohistochemistry Western Blot ELISA Immunofluorescence Primary Antibodies By Organ Brain Cancer
Type	Antibody Polyclonal
Format	Liquid
Applications	WB, IF, ELISA, IHC-P
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	APP Antibody: AAA, AD1, PN2, ABPP, APPI, CVAP, ABETA, PN-II, CTFgamma, A4, Amyloid beta A4 protein, APP
Available
Shipping	blue ice or RT
By Research Area	Neuroscience
Homology	Predicted species reactivity based on immunogen sequence: Pig: (100%), Guinea pig: (100%)
Immunogen	APP antibody was raised against a 15 amino acid peptide near the carboxy terminus of human APP. The immunogen is located within the last 50 amino acids of APP.
Applications	APP antibody can be used for detection of APP and the C99 fragment by Western blot at 0.5 - 1 μ g/mL. Antibody can also be used for immunohistochemistry starting at 2 μ g/mL. For immunofluorescence start at 20 μ g/mL. Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.
Positive Control 1	Cat. No. 1403 - Mouse Brain Tissue Lysate
Predicted Molecular Weight	Predicted: 83 kDa Observed: 110 kDa
Purification	APP Antibody is affinity chromatography purified via peptide column.
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	APP Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/ml
Storage Conditions	APP antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Related Products	Cat. No. 2136P – APP Peptide
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	APP
Accession #	CAA30050
Protein Gi #	28721
Ncbi Gene Id #	351
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	APP
Ncbi Official Full Name	amyloid beta (A4) precursor protein
Ncbi Organism	Homo sapiens
Swissprot #	P05067
Background	APP Antibody: Accumulation of the amyloid-beta peptide (Abeta) in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. The beta-amyloid protein precursor (APP) is cleaved by beta-secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for gamma-secretase to generate the 4 kDa amyloid-beta peptide (Abeta), which is deposited in the brains of all suffers of Alzheimer's disease.
Background References 1	Ponte P, Gonzalez-DeWhitt P, Schilling J, Miller J, Hsu D, Greenberg B, Davis K, Wallace W, Lieberburg I, Fuller F. A new A4 amyloid mRNA contains a domain homologous to serine proteinase inhibitors. Nature 1988; 331:525-7
Background References 2	Selkoe D.J. Cell biology of the amyloid β -protein precursor and the mechanism of Alzheimer's disease.Annu Rev Cell Biol 1994; 10:373-403 (RD1299)
1st Image Caption	Western blot analysis of APP in mouse brain tissue lysate with APP antibody at (A) 0.5 and (B) 1 μ g/ml.
2nd Image Caption	Immunohistochemical staining of rat brain using APP antibody at 2 μ g/mL.
3rd Image Caption	Immunofluorescence of APP in Rat Brain cells with APP antibody at 20 μ g/mL.
4th Image Caption	Immunohistochemistry of APP in mouse brain tissue with APP Antibody at 5 μ g/mL.
5th Image Caption	Immunofluorescence of APP in mouse brain tissue with APP Antibody at 20 μ g/mL. Green: APP antibody (2136) Red: Phylloidin staining Blue: DAPI staining

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 0.1 mg

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