Item no. |
PRS-19-872-50uL |
Manufacturer |
ProSci
|
Amount |
50 uL |
Category |
|
Type |
Antibody Primary |
Format |
Liquid |
Applications |
WB, IP, IHC, CHIP |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
MOT1, TAF(II)17TAF172, TAFII17TATA-binding protein-associated factor 172, ATP-dependent helicase BTAF1, B-TFIID transcription factor-associated 170 kDa subunit, BTAF1 RNA polymerase II, B-TFIID transcription factor-associated, 170kDa (Mot1 homolog, S. cer |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Other |
Immunogen |
Recombinant fusion protein containing a sequence corresponding to amino acids 1600-1849 of human BTAF1 (NP_003963.1). |
Applications |
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IP: 1:20 - 1:50
ChIP: 1:20 - 1:100 |
Positive Control 1 |
HepG2 |
Positive Control 2 |
HeLa |
Positive Control 3 |
MCF7 |
Positive Control 4 |
Rat testis |
Predicted Molecular Weight |
Observed: 250kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
BTAF1 |
Ncbi Gene Id # |
9044 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
BTAF1 |
Ncbi Official Full Name |
B-TFIID TATA-box binding protein associated factor 1 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
O14981 |
Background |
This gene encodes a TAF (TATA box-binding protein-associated factor), which associates with TBP (TATA box-binding protein) to form the B-TFIID complex that is required for transcription initiation of genes by RNA polymerase II. This TAF has DNA-dependent ATPase activity, which drives the dissociation of TBP from DNA, freeing the TBP to associate with other TATA boxes or TATA-less promoters. |
1st Image Caption |
Western blot analysis of extracts of various cell lines, using BTAF1 antibody (19-872) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. |
2nd Image Caption |
Immunoprecipitation analysis of 150ug extracts of HeLa cells using 3ug BTAF1 antibody (19-872). Western blot was performed from the immunoprecipitate using BTAF1 antibody (19-872) at a dilition of 1:500. |
3rd Image Caption |
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using BTAF1 antibody (19-872) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.