Item no. |
PRS-19-156-50uL |
Manufacturer |
ProSci
|
Amount |
50 uL |
Category |
|
Type |
Antibody Primary |
Format |
Liquid |
Applications |
WB, IF |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
ATPDase, CD39, NTPDase-1, SPG64, ectonucleoside triphosphate diphosphohydrolase 1, CD39 antigen, ecto-ATP diphosphohydrolase 1, ecto-ATPDase 1, ecto-ATPase 1, ecto-apyrase, lymphoid cell activation antigen |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Immunology |
Immunogen |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human ENTPD1 (NP_001157655.1). |
Applications |
WB: 1:200 - 1:1000
IF: 1:50 - 1:200 |
Positive Control 1 |
U-251MG |
Positive Control 2 |
HeLa |
Positive Control 3 |
A-549 |
Positive Control 4 |
LO2 |
Positive Control 5 |
HL-60 |
Positive Control 6 |
MCF7 |
Predicted Molecular Weight |
Observed: 65kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
ENTPD1 |
Ncbi Gene Id # |
953 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
ENTPD1 |
Ncbi Official Full Name |
ectonucleoside triphosphate diphosphohydrolase 1 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P49961 |
Background |
The protein encoded by this gene is a plasma membrane protein that hydrolyzes extracellular ATP and ADP to AMP. Inhibition of this protein's activity may confer anticancer benefits. Several transcript variants encoding different isoforms have been found for this gene. |
1st Image Caption |
Western blot analysis of extracts of various cell lines, using ENTPD1 antibody (19-156) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s. |
2nd Image Caption |
Immunofluorescence analysis of Raw264.7 cells using ENTPD1 Polyclonal Antibody (19-156) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
3rd Image Caption |
Immunofluorescence analysis of THP-1 cells using ENTPD1 Polyclonal Antibody (19-156) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
4th Image Caption |
Immunofluorescence analysis of human placenta using ENTPD1 Polyclonal Antibody (19-156) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.