« Back
Home /
ACO2 Antibody

ACO2 Antibody

Item no.	PRS-19-131-50uL
Manufacturer	ProSci
Amount	50 uL
Category	Antibodies Western Blot Immunofluorescence Primary Antibodies
Type	Antibody Primary
Format	Liquid
Applications	WB, IF
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	ACO2, ACONM, MGC20605, MGC33908, ICRD
Available
Shipping	blue ice or RT
By Research Area	Cancer, Signal Transduction
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 501-780 of human ACO2 (NP_001089.1).
Applications	WB: 1:500 - 1:2000 IF: 1:50 - 1:200
Positive Control 1	HeLa
Positive Control 2	U-87MG
Positive Control 3	K-562
Positive Control 4	NIH/3T3
Positive Control 5	Mouse brain
Positive Control 6	Rat spinal cord
Predicted Molecular Weight	Observed: 105kDa
Purification	Affinity purification
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions	Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	ACO2
Ncbi Gene Id #	50
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	ACO2
Ncbi Official Full Name	aconitase 2, mitochondrial
Ncbi Organism	Homo sapiens
Swissprot #	Q99798
Background	The protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification.
1st Image Caption	Western blot analysis of extracts of various cell lines, using ACO2 antibody (19-131) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
2nd Image Caption	Immunofluorescence analysis of HeLa cells using ACO2 antibody (19-131) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

Request product datasheet

Request safety datasheet

Simple Order Order Subscription

Amount: 50 uL

available

Delivery expected until 9/6/2024

Express Delivery: Save 1 days

Compare

Add to wishlist

Recommend by mail