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MAP1B Antibody

MAP1B Antibody

Item no.	PRS-19-029-50uL
Manufacturer	ProSci
Amount	50 uL
Category	Neuroscience Neural Lineage Markers Antibodies Immunohistochemistry Western Blot Immunofluorescence Primary Antibodies Glial Cell Markers
Type	Antibody Primary
Format	Liquid
Applications	WB, IF, IHC
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	MAP1B, FUTSCH, MAP-1B, MAP5
Available
Shipping	blue ice or RT
By Research Area	Cell Cycle, Neuroscience, Signal Transduction
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1820-2100 of human MAP1B (NP_005900.2).
Applications	WB: 1:200 - 1:2000 IHC: 1:50 - 1:200 IF: 1:50 - 1:200
Positive Control 1	DU145
Positive Control 2	293T
Positive Control 3	HT-1080
Positive Control 4	Rat testis
Predicted Molecular Weight	Observed: 350kDa
Purification	Affinity purification
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions	Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	MAP1B
Ncbi Gene Id #	4131
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	MAP1B
Ncbi Official Full Name	microtubule-associated protein 1B
Ncbi Organism	Homo sapiens
Swissprot #	P46821
Background	This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The product of this gene is a precursor polypeptide that presumably undergoes proteolytic processing to generate the final MAP1B heavy chain and LC1 light chain. Gene knockout studies of the mouse microtubule-associated protein 1B gene suggested an important role in development and function of the nervous system.
1st Image Caption	Western blot analysis of extracts of various cell lines, using MAP1B antibody (19-029) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
2nd Image Caption	Immunofluorescence analysis of C6 cells using MAP1B antibody (19-029) at dilution of 1:100. Blue: DAPI for nuclear staining.
3rd Image Caption	Immunofluorescence analysis of NIH/3T3 cells using MAP1B antibody (19-029) at dilution of 1:100. Blue: DAPI for nuclear staining.
4th Image Caption	Immunofluorescence analysis of U2OS cells using MAP1B antibody (19-029) at dilution of 1:100. Blue: DAPI for nuclear staining.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 50 uL

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Delivery expected until 10/4/2024

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