Item no. |
PRS-19-029-50uL |
Manufacturer |
ProSci
|
Amount |
50 uL |
Category |
|
Type |
Antibody Primary |
Format |
Liquid |
Applications |
WB, IF, IHC |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
MAP1B, FUTSCH, MAP-1B, MAP5 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Cell Cycle, Neuroscience, Signal Transduction |
Immunogen |
Recombinant fusion protein containing a sequence corresponding to amino acids 1820-2100 of human MAP1B (NP_005900.2). |
Applications |
WB: 1:200 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200 |
Positive Control 1 |
DU145 |
Positive Control 2 |
293T |
Positive Control 3 |
HT-1080 |
Positive Control 4 |
Rat testis |
Predicted Molecular Weight |
Observed: 350kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
MAP1B |
Ncbi Gene Id # |
4131 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
MAP1B |
Ncbi Official Full Name |
microtubule-associated protein 1B |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P46821 |
Background |
This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The product of this gene is a precursor polypeptide that presumably undergoes proteolytic processing to generate the final MAP1B heavy chain and LC1 light chain. Gene knockout studies of the mouse microtubule-associated protein 1B gene suggested an important role in development and function of the nervous system. |
1st Image Caption |
Western blot analysis of extracts of various cell lines, using MAP1B antibody (19-029) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s. |
2nd Image Caption |
Immunofluorescence analysis of C6 cells using MAP1B antibody (19-029) at dilution of 1:100. Blue: DAPI for nuclear staining. |
3rd Image Caption |
Immunofluorescence analysis of NIH/3T3 cells using MAP1B antibody (19-029) at dilution of 1:100. Blue: DAPI for nuclear staining. |
4th Image Caption |
Immunofluorescence analysis of U2OS cells using MAP1B antibody (19-029) at dilution of 1:100. Blue: DAPI for nuclear staining. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.