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PDCD4 Antibody, KO Validated

PDCD4 Antibody, KO Validated

Item no.	PRS-18-717-50uL
Manufacturer	ProSci
Amount	50 uL
Category	Oncology Neuroscience Targets for Rodent Neurosciences Antibodies Cell Biology Cell Status Apoptosis Western Blot Primary Antibodies
Type	Antibody Primary
Format	Liquid
Applications	WB
Specific against	Human, Mouse
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	PDCD4 Antibody: H731, H731, Programmed cell death protein 4, Neoplastic transformation inhibitor protein
Available
Shipping	blue ice or RT
By Research Area	Apoptosis, Cancer, Cell Cycle, Signal Transduction
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human PDCD4 (NP_055271.2).
Applications	WB: 1:500 - 1:2000
Positive Control 1	MCF-7
Positive Control 2	HeLa
Positive Control 3	HepG2
Positive Control 4	Mouse thymus
Predicted Molecular Weight	Observed: 60kDa
Validation	Antibody is Knockout validated.
Purification	Affinity purification
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions	Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	PDCD4
Ncbi Gene Id #	27250
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	PDCD4
Ncbi Official Full Name	programmed cell death 4 (neoplastic transformation inhibitor)
Ncbi Organism	Homo sapiens
Swissprot #	Q53EL6
Background	This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants.
1st Image Caption	Western blot analysis of extracts of various cell lines, using PDCD4 antibody (18-717) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
2nd Image Caption	Western blot analysis of extracts from normal (control) and PDCD4 knockout (KO) HeLa cells, using PDCD4 antibody (18-717) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 50 uL

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Delivery expected until 9/6/2024

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