Item no. |
PRS-18-652-100uL |
Manufacturer |
ProSci
|
Amount |
100 uL |
Category |
|
Type |
Antibody Primary |
Format |
Liquid |
Applications |
WB, FC, IHC |
Specific against |
Human, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Interleukin-6, IL-6, B-cell stimulatory factor 2, BSF-2, CTL differentiation factor, CDF, Hybridoma growth factor, Interferon beta-2, IFN-beta-2, IL6, IFNB2 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Apoptosis, Cancer, Cell Cycle, Chemokines & Cytokines, Growth Factors, Immunology, Innate Immunity, Neuroscience, Obesity |
Immunogen |
A synthetic peptide of human IL6 |
Applications |
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
Flow: 1:20 - 1:50 |
Positive Control 1 |
NCI-H460 |
Positive Control 2 |
293T |
Positive Control 3 |
K-562 |
Positive Control 4 |
Rat lung |
Positive Control 5 |
Rat kidney |
Predicted Molecular Weight |
Observed: 26kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, pH7.3. |
Storage Conditions |
Store at 4˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
IL6 |
Ncbi Gene Id # |
3569 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
IL6 |
Ncbi Official Full Name |
interleukin 6 (interferon, beta 2) |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P05231 |
Background |
This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Alternative splicing results in multiple transcript variants. |
1st Image Caption |
Western blot analysis of extracts of various cell lines, using IL6 antibody (18-652) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.