Comparison

IL6 Antibody

Item no. PRS-18-652-100uL
Manufacturer ProSci
Amount 100 uL
Category
Type Antibody Primary
Format Liquid
Applications WB, FC, IHC
Specific against Human, Rat
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias Interleukin-6, IL-6, B-cell stimulatory factor 2, BSF-2, CTL differentiation factor, CDF, Hybridoma growth factor, Interferon beta-2, IFN-beta-2, IL6, IFNB2
Available
Shipping
blue ice or RT
By Research Area
Apoptosis, Cancer, Cell Cycle, Chemokines & Cytokines, Growth Factors, Immunology, Innate Immunity, Neuroscience, Obesity
Immunogen
A synthetic peptide of human IL6
Applications
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

Flow: 1:20 - 1:50
Positive Control 1
NCI-H460
Positive Control 2
293T
Positive Control 3
K-562
Positive Control 4
Rat lung
Positive Control 5
Rat kidney
Predicted Molecular Weight
Observed: 26kDa
Purification
Affinity purification
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Buffer
PBS with 0.02% sodium azide, pH7.3.
Storage Conditions
Store at 4˚ C. Avoid freeze / thaw cycles.
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications
None
Ncbi Official Symbol
IL6
Ncbi Gene Id #
3569
User Note
Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol
IL6
Ncbi Official Full Name
interleukin 6 (interferon, beta 2)
Ncbi Organism
Homo sapiens
Swissprot #
P05231
Background
This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Alternative splicing results in multiple transcript variants.
1st Image Caption
Western blot analysis of extracts of various cell lines, using IL6 antibody (18-652) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 uL
Available: Out of stock
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