Item no. |
PRS-18-522-50uL |
Manufacturer |
ProSci
|
Amount |
50 uL |
Category |
|
Type |
Antibody Primary |
Format |
Liquid |
Applications |
WB |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
HEL-S-5polyubiquitin-B, epididymis secretory protein Li 5polyubiquitin B |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Apoptosis, Cell Cycle, Neuroscience |
Immunogen |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-76 of human Ubiquitin (NP_061828.1). |
Applications |
WB: 1:500 - 1:2000 |
Positive Control 1 |
BT-474 |
Positive Control 2 |
SW480 |
Positive Control 3 |
Mouse kidney |
Positive Control 4 |
Mouse liver |
Positive Control 5 |
Rat brain |
Positive Control 6 |
Rat kidney |
Predicted Molecular Weight |
Observed: 17-110kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
UBB |
Ncbi Gene Id # |
7314 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
UBB |
Ncbi Official Full Name |
ubiquitin B |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P0CG47 |
Background |
This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. An aberrant form of this protein has been detected in patients with Alzheimer's disease and Down syndrome. Pseudogenes of this gene are located on chromosomes 1, 2, 13, and 17. Alternative splicing results in multiple transcript variants. |
1st Image Caption |
Western blot analysis of extracts of various cell lines, using Ubiquitin antibody (18-522) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.