Item no. |
PRS-18-396-50uL |
Manufacturer |
ProSci
|
Amount |
50 uL |
Category |
|
Type |
Antibody Primary |
Format |
Liquid |
Applications |
WB, IHC |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
GPX4, glutathione peroxidase 4 (phospholipid hydroperoxidase), HGNC:4556, PHGPx, snGPx, glutathione peroxidase 4, phospholipid hydroperoxidase, sperm nucleus glutathione peroxidase, MCSP, GPx-4, GSHPx-4, snPHGPx |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Cancer, Signal Transduction |
Immunogen |
Recombinant fusion protein containing a sequence corresponding to amino acids 30-197 of human GPX4 (NP_002076.2). |
Applications |
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200 |
Positive Control 1 |
Mouse lung |
Positive Control 2 |
Rat testis |
Predicted Molecular Weight |
Observed: 25kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
GPX4 |
Ncbi Gene Id # |
2879 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
GPX4 |
Ncbi Official Full Name |
glutathione peroxidase 4 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P36969 |
Background |
The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of hydrogen peroxide, organic hydroperoxides and lipid hydroperoxides, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme has a high preference for lipid hydroperoxides and protects cells against membrane lipid peroxidation and cell death. It is also required for normal sperm development; thus, it has been identified as a 'moonlighting' protein because of its ability to serve dual functions as a peroxidase, as well as a structural protein in mature spermatozoa. Mutations in this gene are associated with Sedaghatian type of spondylometaphyseal dysplasia (SMDS). This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene. |
1st Image Caption |
Western blot analysis of extracts of various cell lines, using GPX4 antibody (18-396) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s. |
2nd Image Caption |
Immunohistochemistry of paraffin-embedded human esophageal cancer using GPX4 Antibody (18-396) at dilution of 1:200 (40x lens). |
3rd Image Caption |
Immunohistochemistry of paraffin-embedded mouse spleen using GPX4 Antibody (18-396) at dilution of 1:200 (40x lens). |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.