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PTPN2 Antibody

PTPN2 Antibody

Item no.	PRS-18-270-50uL
Manufacturer	ProSci
Amount	50 uL
Category	Antibodies Immunohistochemistry Western Blot Immunofluorescence Primary Antibodies
Type	Antibody Primary
Format	Liquid
Applications	WB, IF, IHC
Specific against	Human, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	PTPN2, PTPT, TC-PTP, TCELLPTP, TCPTP, PTN2
Available
Shipping	blue ice or RT
By Research Area	Immunology, Signal Transduction
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-353 of human PTPN2 (NP_536348.1).
Applications	WB: 1:500 - 1:2000 IHC: 1:50 - 1:200 IF: 1:50 - 1:200
Positive Control 1	SW480
Positive Control 2	BT-474
Positive Control 3	Jurkat
Positive Control 4	K-562
Predicted Molecular Weight	Observed: 45kDa
Purification	Affinity purification
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions	Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	PTPN2
Ncbi Gene Id #	5771
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	PTPN2
Ncbi Official Full Name	protein tyrosine phosphatase, non-receptor type 2
Ncbi Organism	Homo sapiens
Swissprot #	P17706
Background	The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. Epidermal growth factor receptor and the adaptor protein Shc were reported to be substrates of this PTP, which suggested the roles in growth factor mediated cell signaling. Multiple alternatively spliced transcript variants encoding different isoforms have been found. Two highly related but distinctly processed pseudogenes that localize to chromosomes 1 and 13, respectively, have been reported.
1st Image Caption	Western blot analysis of extracts of various cell lines, using PTPN2 antibody (18-270) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.
2nd Image Caption	Immunohistochemistry of paraffin-embedded rat heart using PTPN2 antibody (18-270) at dilution of 1:100 (40x lens).
3rd Image Caption	Immunofluorescence analysis of MCF-7 cells using PTPN2 antibody (18-270). Blue: DAPI for nuclear staining.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 50 uL

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Delivery expected until 9/6/2024

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