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KRT20 Antibody, KO Validated

KRT20 Antibody, KO Validated

Item no.	PRS-18-183-50uL
Manufacturer	ProSci
Amount	50 uL
Category	Oncology Antibodies Immunohistochemistry Western Blot Immunofluorescence Primary Antibodies By Organ Bladder Cancer Gastric Cancer
Type	Antibody Primary
Format	Liquid
Applications	WB, IF, IHC
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	KRT20, CD20, CK20, K20, KRT21, MGC35423, CK-20
Available
Shipping	blue ice or RT
By Research Area	Cancer, Cell Cycle, Signal Transduction
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 245-424 of human KRT20 (NP_061883.1).
Applications	WB: 1:500 - 1:2000 IHC: 1:50 - 1:200 IF: 1:50 - 1:200
Positive Control 1	HeLa
Predicted Molecular Weight	Observed: 48kDa
Validation	Antibody is Knockout validated.
Purification	Affinity purification
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions	Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	KRT20
Ncbi Gene Id #	54474
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	KRT20
Ncbi Official Full Name	keratin 20
Ncbi Organism	Homo sapiens
Swissprot #	P35900
Background	The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21.
1st Image Caption	Western blot analysis of extracts from normal (control) and KRT20 knockout (KO) HeLa cells, using KRT20 antibody (18-183) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 50 uL

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Delivery expected until 9/6/2024

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