Item no. |
PRS-18-119-50uL |
Manufacturer |
ProSci
|
Amount |
50 uL |
Category |
|
Type |
Antibody Primary |
Format |
Liquid |
Applications |
WB, IHC |
Specific against |
Mouse, Rat, Duck |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
CTSK, cathepsin K, CTS02, CTSO, CTSO1, CTSO2, MGC23107, PKND, PYCD, cathepsin K (pycnodysostosis), cathepsin O1, cathepsin O2, cathepsin X |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Cancer, Cell Cycle, Neuroscience, Signal Transduction |
Immunogen |
Recombinant fusion protein containing a sequence corresponding to amino acids 115-329 of human CTSK (NP_000387.1). |
Applications |
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100 |
Positive Control 1 |
Mouse liver |
Predicted Molecular Weight |
Observed: 41kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
CTSK |
Ncbi Gene Id # |
1513 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
CTSK |
Ncbi Official Full Name |
cathepsin K |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P43235 |
Background |
The protein encoded by this gene is a lysosomal cysteine proteinase involved in bone remodeling and resorption. This protein, which is a member of the peptidase C1 protein family, is predominantly expressed in osteoclasts. However, the encoded protein is also expressed in a significant fraction of human breast cancers, where it could contribute to tumor invasiveness. Mutations in this gene are the cause of pycnodysostosis, an autosomal recessive disease characterized by osteosclerosis and short stature. |
1st Image Caption |
Western blot analysis of extracts of mouse liver, using CTSK antibody (18-119) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s. |
2nd Image Caption |
Immunohistochemistry of paraffin-embedded mouse brain using CTSK antibody (18-119) at dilution of 1:100 (40x lens). |
3rd Image Caption |
Immunohistochemistry of paraffin-embedded rat brain using CTSK antibody (18-119) at dilution of 1:100 (40x lens). |
4th Image Caption |
Immunohistochemistry of paraffin-embedded mouse lung using CTSK antibody (18-119) at dilution of 1:100 (40x lens). |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.