Comparison

IGF2BP2 Antibody

Item no. PRS-18-082-50uL
Manufacturer ProSci
Amount 50 uL
Category
Type Antibody Primary
Format Liquid
Applications WB, IF, IHC
Specific against Human, Mouse, Rat
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias insulin-like growth factor 2 mRNA binding protein 2, IMP-2, IMP2, VICKZ2, p62, IGF II mRNA binding protein 2, IGF-II mRNA-binding protein 2
Available
Shipping
blue ice or RT
By Research Area
Cell Cycle, Growth Factors, Immunology, Signal Transduction
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 330-599 of human IGF2BP2 (NP_006539.3).
Applications
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Positive Control 1
HT-29
Positive Control 2
BxPC-3
Positive Control 3
HeLa
Positive Control 4
Jurkat
Positive Control 5
NIH/3T3
Predicted Molecular Weight
Observed: 70kDa
Purification
Affinity purification
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions
Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications
None
Ncbi Official Symbol
IGF2BP2
Ncbi Gene Id #
10644
User Note
Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol
IGF2BP2
Ncbi Official Full Name
insulin-like growth factor 2 mRNA binding protein 2
Ncbi Organism
Homo sapiens
Swissprot #
Q9Y6M1
Background
This gene encodes a member of the IGF-II mRNA-binding protein (IMP) family. The protein encoded by this gene contains four KH domains and two RRM domains. It functions by binding to the 5' UTR of the insulin-like growth factor 2 (IGF2) mRNA and regulating IGF2 translation. Alternative promoter usage and alternate splicing result in multiple variants encoding different isoforms.
1st Image Caption
Western blot analysis of extracts of various cell lines, using IGF2BP2 antibody (18-082) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
2nd Image Caption
Immunofluorescence analysis of U-2 OS cells using IGF2BP2 antibody (18-082) at dilution of 1:100. Blue: DAPI for nuclear staining.
3rd Image Caption
Immunofluorescence analysis of rat testis cells using IGF2BP2 antibody (18-082) at dilution of 1:100. Blue: DAPI for nuclear staining.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 50 uL
Available: In stock
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