Item no. |
PRS-18-082-50uL |
Manufacturer |
ProSci
|
Amount |
50 uL |
Category |
|
Type |
Antibody Primary |
Format |
Liquid |
Applications |
WB, IF, IHC |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
insulin-like growth factor 2 mRNA binding protein 2, IMP-2, IMP2, VICKZ2, p62, IGF II mRNA binding protein 2, IGF-II mRNA-binding protein 2 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Cell Cycle, Growth Factors, Immunology, Signal Transduction |
Immunogen |
Recombinant fusion protein containing a sequence corresponding to amino acids 330-599 of human IGF2BP2 (NP_006539.3). |
Applications |
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
IF: 1:50 - 1:100 |
Positive Control 1 |
HT-29 |
Positive Control 2 |
BxPC-3 |
Positive Control 3 |
HeLa |
Positive Control 4 |
Jurkat |
Positive Control 5 |
NIH/3T3 |
Predicted Molecular Weight |
Observed: 70kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
IGF2BP2 |
Ncbi Gene Id # |
10644 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
IGF2BP2 |
Ncbi Official Full Name |
insulin-like growth factor 2 mRNA binding protein 2 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q9Y6M1 |
Background |
This gene encodes a member of the IGF-II mRNA-binding protein (IMP) family. The protein encoded by this gene contains four KH domains and two RRM domains. It functions by binding to the 5' UTR of the insulin-like growth factor 2 (IGF2) mRNA and regulating IGF2 translation. Alternative promoter usage and alternate splicing result in multiple variants encoding different isoforms. |
1st Image Caption |
Western blot analysis of extracts of various cell lines, using IGF2BP2 antibody (18-082) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s. |
2nd Image Caption |
Immunofluorescence analysis of U-2 OS cells using IGF2BP2 antibody (18-082) at dilution of 1:100. Blue: DAPI for nuclear staining. |
3rd Image Caption |
Immunofluorescence analysis of rat testis cells using IGF2BP2 antibody (18-082) at dilution of 1:100. Blue: DAPI for nuclear staining. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.