Comparison

PIK3R3 Antibody

Item no. PRS-16-869-50uL
Manufacturer ProSci
Amount 50 uL
Category
Type Antibody Primary
Format Liquid
Applications WB, IF, IHC
Specific against Human, Mouse, Rat
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias PIK3R3, DKFZp686P05226, FLJ41892, p55-GAMMA, p55
Available
Shipping
blue ice or RT
By Research Area
Apoptosis, Cell Cycle, Immunology, Signal Transduction
Immunogen
Recombinant protein of human PIK3R3.
Applications
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Positive Control 1
Mouse brain
Predicted Molecular Weight
Observed: 54kDa
Purification
Affinity purification
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions
Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications
None
Ncbi Official Symbol
PIK3R3
Ncbi Gene Id #
8503
User Note
Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol
PIK3R3
Ncbi Official Full Name
phosphoinositide-3-kinase, regulatory subunit 3 (gamma)
Ncbi Organism
Homo sapiens
Swissprot #
Q92569
Background
Phosphatidylinositol 3-kinase (PI3K) phosphorylates phosphatidylinositol and similar compounds, which then serve as second messengers in growth signaling pathways. PI3K is composed of a catalytic and a regulatory subunit. The protein encoded by this gene represents a regulatory subunit of PI3K. The encoded protein contains two SH2 domains through which it binds activated protein tyrosine kinases to regulate their activity.
1st Image Caption
Western blot analysis of extracts of Mouse brain, using PIK3R3 antibody (16-869) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
2nd Image Caption
Immunofluorescence analysis of NIH-3T3 cells using PIK3R3 antibody (16-869) at dilution of 1:100. Blue: DAPI for nuclear staining.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 50 uL
Available: In stock
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