Comparison

PPAR gamma Antibody

Item no. PRS-16-812-50uL
Manufacturer ProSci
Amount 50 uL
Category
Type Antibody Primary
Format Liquid
Applications WB
Specific against Human, Mouse, Rat
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias PPARG, GLM1, CIMT1, NR1C3, PPARG1, PPARG2, PPARgamma
Available
Shipping
blue ice or RT
By Research Area
Cell Cycle, Neuroscience, Obesity, Signal Transduction, Stem Cell, Transcription
Immunogen
Recombinant protein of human PPAR gamma
Applications
WB: 1:500 - 1:2000
Positive Control 1
SW620
Positive Control 2
rat lung
Predicted Molecular Weight
Observed: 58kDa
Purification
Affinity purification
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions
Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications
None
Ncbi Official Symbol
PPARG
Ncbi Gene Id #
5468
User Note
Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol
PPARG
Ncbi Official Full Name
peroxisome proliferator-activated receptor gamma
Ncbi Organism
Homo sapiens
Swissprot #
P37231
Background
This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described.
1st Image Caption
Western blot analysis of extracts of various cell lines, using PPAR gamma antibody (16-812) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 180s.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 50 uL
Available: In stock
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