CDK4 Antibody

Cancer, Cell Cycle, Signal Transduction

WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200

IP: 1:50 - 1:200

293T

Observed: 34kDa

Polyclonal

Unconjugated

Store at -20˚ C. Avoid freeze / thaw cycles.

None

1019

CDK4

Homo sapiens

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported.

Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug CDK4 antibody (16-759). Western blot was performed from the immunoprecipitate using CDK4 antibody (16-759) at a dilition of 1:1000.