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XRCC4 Antibody

XRCC4 Antibody

Item no.	PRS-16-747-50uL
Manufacturer	ProSci
Amount	50 uL
Category	Oncology Antibodies By Cell Type Carcinoma Immunohistochemistry Western Blot Immunofluorescence Primary Antibodies
Type	Antibody Primary
Format	Liquid
Applications	WB, IF, IHC
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	DNA repair protein XRCC4, X-ray repair cross-complementing protein 4, XRCC4
Available
Shipping	blue ice or RT
By Research Area	Apoptosis, Cell Cycle
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-336 of human XRCC4 (NP_071801.1).
Applications	WB: 1:500 - 1:2000 IHC: 1:50 - 1:200 IF: 1:10 - 1:100
Positive Control 1	Jurkat
Positive Control 2	MCF7
Positive Control 3	K562
Positive Control 4	HeLa
Positive Control 5	mouse thymus
Positive Control 6	mouse heart
Predicted Molecular Weight	Observed: 55kDa
Purification	Affinity purification
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions	Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	XRCC4
Ncbi Gene Id #	7518
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	XRCC4
Ncbi Official Full Name	DNA repair protein XRCC4
Ncbi Organism	Homo sapiens
Swissprot #	Q13426
Background	The protein encoded by this gene functions together with DNA ligase IV and the DNA-dependent protein kinase in the repair of DNA double-strand breaks. This protein plays a role in both non-homologous end joining and the completion of V(D)J recombination. Mutations in this gene can cause short stature, microcephaly, and endocrine dysfunction (SSMED). Alternative splicing generates several transcript variants.
1st Image Caption	Western blot analysis of extracts of various cell lines, using XRCC4 antibody (16-747) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
2nd Image Caption	Immunohistochemistry of paraffin-embedded human appendix using XRCC4 antibody (16-747) at dilution of 1:100 (40x lens).
3rd Image Caption	Immunohistochemistry of paraffin-embedded human breast cancer using XRCC4 antibody (16-747) at dilution of 1:100 (40x lens).
4th Image Caption	Immunohistochemistry of paraffin-embedded human gastric cancer using XRCC4 antibody (16-747) at dilution of 1:100 (40x lens).
5th Image Caption	Immunofluorescence analysis of 293T cells using XRCC4 antibody (16-747) at dilution of 1:100. Blue: DAPI for nuclear staining.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 50 uL

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Delivery expected until 9/6/2024

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