Item no. |
PRS-16-486-50uL |
Manufacturer |
ProSci
|
Amount |
50 uL |
Category |
|
Type |
Antibody Primary |
Format |
Liquid |
Applications |
WB, IF, IP, IHC, CHIP |
Specific against |
Human, Mouse, Rat |
Host |
Mouse |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
H3F3A, H3t, H3.4, H3/g, H3FT |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Signal Transduction |
Immunogen |
A synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3 |
Applications |
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:50 - 1:200
ChIP: 1:20 - 1:100 |
Purification |
Affinity purification |
Clonality |
Monoclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
Methylated |
Ncbi Official Symbol |
H3K27me3 |
Ncbi Gene Id # |
8290 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
H3K27me3 |
Ncbi Official Full Name |
histone cluster 3, H3 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q16695 |
Background |
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. |
1st Image Caption |
Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K27 antibody (16-486) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s. |
2nd Image Caption |
Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K27 antibody (16-486) at 1:1000 dilution. |
3rd Image Caption |
Immunohistochemistry of paraffin-embedded human colon carcinoma using TriMethyl-Histone H3-K27 antibody (16-486) at dilution of 1:25 (40x lens). |
4th Image Caption |
Immunohistochemistry of paraffin-embedded mouse brain using TriMethyl-Histone H3-K27 antibody (16-486) at dilution of 1:25 (40x lens). |
5th Image Caption |
Immunohistochemistry of paraffin-embedded rat brain using TriMethyl-Histone H3-K27 antibody (16-486) at dilution of 1:25 (40x lens). |
6th Image Caption |
Immunofluorescence analysis of C6 cells using TriMethyl-Histone H3-K27 antibody (16-486) at dilution of 1:25. Blue: DAPI for nuclear staining. |
7th Image Caption |
Immunofluorescence analysis of NIH/3T3 cells using TriMethyl-Histone H3-K27 antibody (16-486) at dilution of 1:25. Blue: DAPI for nuclear staining. |
8th Image Caption |
Immunofluorescence analysis of U-2OS cells using TriMethyl-Histone H3-K27 antibody (16-486) at dilution of 1:25. Blue: DAPI for nuclear staining. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.