Item no. |
PRS-16-303-50uL |
Manufacturer |
ProSci
|
Amount |
50 uL |
Category |
|
Type |
Antibody Primary |
Format |
Liquid |
Applications |
WB |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
CERK, Acylsphingosine kinase, Ceramide kinase, DA59H18.2, DA59H18.3, KIAA1646, Lipid kinase 4, Lipid kinase LK4, HCERK, LK4 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Apoptosis, Cell Cycle, Signal Transduction |
Immunogen |
A synthetic peptide corresponding to a sequence within amino acids 400 to the C-terminus of human CERK (NP_073603.2). |
Applications |
WB: 1:500 - 1:2000 |
Positive Control 1 |
LO2 |
Positive Control 2 |
A-549 |
Positive Control 3 |
Mouse testis |
Positive Control 4 |
Rat thymus |
Predicted Molecular Weight |
Observed: 60kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
CERK |
Ncbi Gene Id # |
64781 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
CERK |
Ncbi Official Full Name |
ceramide kinase |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q8TCT0 |
Background |
CERK converts ceramide to ceramide 1-phosphate (C1P), a sphingolipid metabolite. Both CERK and C1P have been implicated in various cellular processes, including proliferation, apoptosis, phagocytosis, and inflammation (Kim et al., 2006 [PubMed 16488390]). |
1st Image Caption |
Western blot analysis of extracts of various cell lines, using CERK antibody (16-303) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.