Item no. |
PRS-16-206-50uL |
Manufacturer |
ProSci
|
Amount |
50 uL |
Category |
|
Type |
Antibody Primary |
Format |
Liquid |
Applications |
WB, IHC |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
TPI1, MGC88108, TPI, TIM, TPID, HEL-S-49 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Cancer, Signal Transduction |
Immunogen |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-249 of human TPI1 (NP_000356.1). |
Applications |
WB: 1:500 - 1:2000
IHC: 1:100 - 1:200 |
Positive Control 1 |
U-87MG |
Positive Control 2 |
293T |
Positive Control 3 |
DU145 |
Positive Control 4 |
A-431 |
Positive Control 5 |
Mouse stomach |
Positive Control 6 |
Mouse brain |
Predicted Molecular Weight |
Observed: 30kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
TPI1 |
Ncbi Gene Id # |
7167 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
TPI1 |
Ncbi Official Full Name |
triosephosphate isomerase 1 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P60174 |
Background |
This gene encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants. |
1st Image Caption |
Western blot analysis of extracts of various cell lines, using TPI1 antibody (16-206) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.