Comparison

TPI1 Antibody

Item no. PRS-16-206-50uL
Manufacturer ProSci
Amount 50 uL
Category
Type Antibody Primary
Format Liquid
Applications WB, IHC
Specific against Human, Mouse, Rat
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias TPI1, MGC88108, TPI, TIM, TPID, HEL-S-49
Available
Shipping
blue ice or RT
By Research Area
Cancer, Signal Transduction
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-249 of human TPI1 (NP_000356.1).
Applications
WB: 1:500 - 1:2000

IHC: 1:100 - 1:200
Positive Control 1
U-87MG
Positive Control 2
293T
Positive Control 3
DU145
Positive Control 4
A-431
Positive Control 5
Mouse stomach
Positive Control 6
Mouse brain
Predicted Molecular Weight
Observed: 30kDa
Purification
Affinity purification
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions
Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications
None
Ncbi Official Symbol
TPI1
Ncbi Gene Id #
7167
User Note
Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol
TPI1
Ncbi Official Full Name
triosephosphate isomerase 1
Ncbi Organism
Homo sapiens
Swissprot #
P60174
Background
This gene encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants.
1st Image Caption
Western blot analysis of extracts of various cell lines, using TPI1 antibody (16-206) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 50 uL
Available: In stock
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