Item no. |
PRS-16-031-50uL |
Manufacturer |
ProSci
|
Amount |
50 uL |
Category |
|
Type |
Antibody Primary |
Format |
Liquid |
Applications |
WB, IHC |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
TNFRSF1A, tumor necrosis factor-alpha receptor, tumor necrosis factor receptor type 1, tumor necrosis factor receptor superfamily, member 1A, tumor necrosis factor receptor superfamily member 1A, tumor necrosis factor receptor 1A isoform beta, tumor necro |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Apoptosis, Cell Cycle, Chemokines & Cytokines, Growth Factors, Immunology, Innate Immunity, Neuroscience, Signal Transduction |
Immunogen |
Recombinant fusion protein containing a sequence corresponding to amino acids 22-211 of human TNFR1 (NP_001056.1). |
Applications |
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200 |
Positive Control 1 |
A-549 |
Positive Control 2 |
Mouse lung |
Predicted Molecular Weight |
Observed: 56kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
TNFRSF1A |
Ncbi Gene Id # |
7132 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
TNFRSF1A |
Ncbi Official Full Name |
tumor necrosis factor receptor superfamily, member 1A |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P19438 |
Background |
This gene encodes a member of the TNF receptor superfamily of proteins. The encoded receptor is found in membrane-bound and soluble forms that interact with membrane-bound and soluble forms, respectively, of its ligand, tumor necrosis factor alpha. Binding of membrane-bound tumor necrosis factor alpha to the membrane-bound receptor induces receptor trimerization and activation, which plays a role in cell survival, apoptosis, and inflammation. Proteolytic processing of the encoded receptor results in release of the soluble form of the receptor, which can interact with free tumor necrosis factor alpha to inhibit inflammation. Mutations in this gene underlie tumor necrosis factor receptor-associated periodic syndrome (TRAPS), characterized by fever, abdominal pain and other features. Mutations in this gene may also be associated with multiple sclerosis in human patients. |
1st Image Caption |
Western blot analysis of extracts of various cell lines, using TNFR1 antibody (16-031) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s. |
2nd Image Caption |
Immunohistochemistry of paraffin-embedded human stomach using TNFR1 antibody (16-031) at dilution of 1:100 (40x lens). |
3rd Image Caption |
Immunohistochemistry of paraffin-embedded rat testis using TNFR1 antibody (16-031) at dilution of 1:100 (40x lens). |
4th Image Caption |
Immunohistochemistry of paraffin-embedded mouse testis using TNFR1 antibody (16-031) at dilution of 1:100 (40x lens). |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.