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PABPC1 Antibody

PABPC1 Antibody

Manufacturer	ProSci
Category	Antibodies Immunohistochemistry Western Blot Immunofluorescence Primary Antibodies
Type	Antibody Primary
Specific against	Human, Mouse, Rat
Format	Liquid
Applications	WB, IF, IHC
Amount	50 uL
Host	Rabbit
Item no.	PRS-15-770-50uL
eClass 6.1	32160702
eClass 9.0	32160702
Available
Alias	PABPC1, PAB1, PABP, PABP1, PABPC2, PABPL1
Shipping	blue ice or RT
By Research Area	Other
Immunogen	A synthetic peptide corresponding to a sequence within amino acids 350-450 of human PABPC1 (NP_002559.2).
Applications	WB: 1:500 - 1:2000 IHC: 1:50 - 1:100 IF: 1:50 - 1:100
Positive Control 1	A-549
Positive Control 2	HT-29
Positive Control 3	HeLa
Positive Control 4	Mouse brain
Positive Control 5	Rat lung
Predicted Molecular Weight	Observed: 71kDa
Purification	Affinity purification
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions	Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	PABPC1
Ncbi Gene Id #	26986
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	PABPC1
Ncbi Official Full Name	poly(A) binding protein, cytoplasmic 1
Ncbi Organism	Homo sapiens
Swissprot #	P11940
Background	This gene encodes a poly(A) binding protein. The protein shuttles between the nucleus and cytoplasm and binds to the 3' poly(A) tail of eukaryotic messenger RNAs via RNA-recognition motifs. The binding of this protein to poly(A) promotes ribosome recruitment and translation initiation; it is also required for poly(A) shortening which is the first step in mRNA decay. The gene is part of a small gene family including three protein-coding genes and several pseudogenes.
1st Image Caption	Western blot analysis of extracts of various cell lines, using PABPC1 antibody (15-770) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
2nd Image Caption	Immunofluorescence analysis of L929 cells using PABPC1 Polyclonal Antibody (15-770) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
3rd Image Caption	Immunofluorescence analysis of U-2 OS cells using PABPC1 Polyclonal Antibody (15-770) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Delivery expected until 8/2/2024

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