Item no. |
PRS-15-755-50uL |
Manufacturer |
ProSci
|
Amount |
50 uL |
Category |
|
Type |
Antibody Primary |
Format |
Liquid |
Applications |
WB, IHC |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Serine/threonine-protein kinase tousled-like 1, PKU-beta, Tousled-like kinase 1, TLK1, KIAA0137 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Apoptosis, Cell Cycle, Signal Transduction |
Immunogen |
Recombinant fusion protein containing a sequence corresponding to amino acids 160-230 of human TLK1 (NP_036422.3). |
Applications |
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100 |
Positive Control 1 |
LO2 |
Positive Control 2 |
293T |
Positive Control 3 |
BxPC-3 |
Positive Control 4 |
HeLa |
Positive Control 5 |
Jurkat |
Positive Control 6 |
Mouse testis |
Predicted Molecular Weight |
Observed: 80kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
TLK1 |
Ncbi Gene Id # |
9874 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
TLK1 |
Ncbi Official Full Name |
Serine/threonine-protein kinase tousled-like 1 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q9UKI8 |
Background |
The protein encoded by this gene is a serine/threonine kinase that may be involved in the regulation of chromatin assembly. The encoded protein is only active when it is phosphorylated, and this phosphorylation is cell cycle-dependent, with the maximal activity of this protein coming during S phase. The catalytic activity of this protein is diminished by DNA damage and by blockage of DNA replication. Three transcript variants encoding different isoforms have been found for this gene. |
1st Image Caption |
Western blot analysis of extracts of various cell lines, using TLK1 antibody (15-755) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.