Item no. |
PRS-15-738-50uL |
Manufacturer |
ProSci
|
Amount |
50 uL |
Category |
|
Type |
Antibody Primary |
Format |
Liquid |
Applications |
WB |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
DXS522E, F8A, HAP4factor VIII intron 22 protein, coagulation factor VIII-associated (intronic transcript) 1, cpG island protein, factor VIII associated protein, huntingtin-associated protein 40 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Other |
Immunogen |
A synthetic peptide corresponding to a sequence within amino acids 150-250 of human F8A1 (NP_036283.2). |
Applications |
WB: 1:500 - 1:2000 |
Positive Control 1 |
U-251MG |
Positive Control 2 |
A-549 |
Positive Control 3 |
Mouse spleen |
Positive Control 4 |
Rat lung |
Positive Control 5 |
Rat spleen |
Predicted Molecular Weight |
Observed: 39kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
F8A1 |
Ncbi Gene Id # |
8263 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
F8A1 |
Ncbi Official Full Name |
coagulation factor VIII associated 1 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P23610 |
Background |
This gene is contained entirely within intron 22 of the factor VIII gene; spans less than 2 kb, and is transcribed in the direction opposite of factor VIII. A portion of intron 22 (int22h), containing F8A, is repeated twice extragenically closer to the Xq telomere. Although its function is unknown, the observation that this gene is conserved in the mouse implies it has some function. Unlike factor VIII, this gene is transcribed abundantly in a wide variety of cell types. |
1st Image Caption |
Western blot analysis of extracts of various cell lines, using F8A1 antibody (15-738) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.