Item no. |
PRS-15-725-50uL |
Manufacturer |
ProSci
|
Amount |
50 uL |
Category |
|
Type |
Antibody Primary |
Format |
Liquid |
Applications |
WB, IP |
Specific against |
Human, Mouse |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
NR1D1, EAR1, THRA1, THRAL, ear-1, hRev |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Cell Cycle, Growth Factors, Neuroscience, Signal Transduction, Transcription |
Immunogen |
Recombinant protein of human NR1D1 |
Applications |
WB: 1:500 - 1:2000
IP: 1:20 - 1:50 |
Positive Control 1 |
A-549 |
Predicted Molecular Weight |
Observed: 67kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
NR1D1 |
Ncbi Gene Id # |
9572 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
NR1D1 |
Ncbi Official Full Name |
nuclear receptor subfamily 1, group D, member 1 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P20393 |
Background |
This gene encodes a transcription factor that is a member of the nuclear receptor subfamily 1. The encoded protein is a ligand-sensitive transcription factor that negatively regulates the expression of core clock proteins. In particular this protein represses the circadian clock transcription factor aryl hydrocarbon receptor nuclear translocator-like protein 1 (ARNTL). This protein may also be involved in regulating genes that function in metabolic, inflammatory and cardiovascular processes. |
1st Image Caption |
Western blot analysis of extracts of A-549 cells, using NR1D1 antibody (15-725). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.