Item no. |
PRS-15-588-50uL |
Manufacturer |
ProSci
|
Amount |
50 uL |
Category |
|
Type |
Antibody Primary |
Format |
Liquid |
Applications |
WB, IHC |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
DLAT, DLTA, PDC-E2, PDCE2 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Cancer, Signal Transduction |
Immunogen |
Recombinant fusion protein containing a sequence corresponding to amino acids 87-270 of human DLAT (NP_001922.2). |
Applications |
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100 |
Positive Control 1 |
HeLa |
Positive Control 2 |
MCF7 |
Positive Control 3 |
HepG2 |
Positive Control 4 |
K-562 |
Positive Control 5 |
Mouse brain |
Positive Control 6 |
Rat brain |
Predicted Molecular Weight |
Observed: 69kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
DLAT |
Ncbi Gene Id # |
1737 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
DLAT |
Ncbi Official Full Name |
dihydrolipoamide S-acetyltransferase |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P10515 |
Background |
This gene encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure. Mutations in this gene are also a cause of pyruvate dehydrogenase E2 deficiency which causes primary lactic acidosis in infancy and early childhood. |
1st Image Caption |
Western blot analysis of extracts of various cell lines, using DLAT antibody (15-588) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.