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MRPL13 Antibody

MRPL13 Antibody

Item no.	PRS-15-382-50uL
Manufacturer	ProSci
Amount	50 uL
Category	Antibodies Immunohistochemistry Western Blot Primary Antibodies
Type	Antibody Primary
Format	Liquid
Applications	WB, IHC
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	L13, L13A, L13mt, RPL13, RPML13, 39S ribosomal protein L13, mitochondrial, MRP-L13, mitochondrial large ribosomal subunit protein uL13m
Available
Shipping	blue ice or RT
By Research Area	Other
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-179 of human MRPL13 (NP_054797.2).
Applications	WB: 1:500 - 1:2000 IHC: 1:100 - 1:200
Positive Control 1	SH-SY5Y
Positive Control 2	HL-60
Positive Control 3	HT-29
Positive Control 4	A-549
Positive Control 5	HeLa
Positive Control 6	Mouse kidney
Predicted Molecular Weight	Observed: 20kDa
Purification	Affinity purification
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions	Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	MRPL13
Ncbi Gene Id #	28998
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	MRPL13
Ncbi Official Full Name	mitochondrial ribosomal protein L13
Ncbi Organism	Homo sapiens
Swissprot #	Q9BYD1
Background	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein.
1st Image Caption	Western blot analysis of extracts of various cell lines, using MRPL13 antibody (15-382) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 50 uL

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Delivery expected until 10/4/2024

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