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BEST1 Antibody

BEST1 Antibody

Item no.	PRS-15-355-50uL
Manufacturer	ProSci
Amount	50 uL
Category	Antibodies Western Blot Immunofluorescence Primary Antibodies
Type	Antibody Primary
Format	Liquid
Applications	WB, IF
Specific against	Human, Mouse
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	ARB, BEST, BMD, Best1V1Delta2, RP5TU15B, VMD2, bestrophin-1, Best disease, vitelliform macular dystrophy protein 2
Available
Shipping	blue ice or RT
By Research Area	Cell Cycle, Neuroscience
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 376-585 of human BEST1 (NP_004174.1).
Applications	WB: 1:500 - 1:2000 IF: 1:50 - 1:200
Positive Control 1	Mouse liver
Positive Control 2	Mouse lung
Predicted Molecular Weight	Observed: 68kDa
Purification	Affinity purification
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions	Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	BEST1
Ncbi Gene Id #	7439
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	BEST1
Ncbi Official Full Name	bestrophin 1
Ncbi Organism	Homo sapiens
Swissprot #	O76090
Background	This gene encodes a member of the bestrophin gene family. This small gene family is characterized by proteins with a highly conserved N-terminus with four to six transmembrane domains. Bestrophins may form chloride ion channels or may regulate voltage-gated L-type calcium-ion channels. Bestrophins are generally believed to form calcium-activated chloride-ion channels in epithelial cells but they have also been shown to be highly permeable to bicarbonate ion transport in retinal tissue. Mutations in this gene are responsible for juvenile-onset vitelliform macular dystrophy (VMD2), also known as Best macular dystrophy, in addition to adult-onset vitelliform macular dystrophy (AVMD) and other retinopathies. Alternative splicing results in multiple variants encoding distinct isoforms.
1st Image Caption	Western blot analysis of extracts of various cell lines, using BEST1 antibody (15-355) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
2nd Image Caption	Immunofluorescence analysis of U2OS cells using BEST1 antibody (15-355). Blue: DAPI for nuclear staining.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 50 uL

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Delivery expected until 9/6/2024

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