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PNP Antibody

PNP Antibody

Manufacturer	ProSci
Category	Oncology Metabolism Regulation of Appetite Antibodies By Cell Type Carcinoma Obesity Western Blot Immunofluorescence Primary Antibodies
Type	Antibody Primary
Specific against	Human, Mouse
Format	Liquid
Applications	WB, IF
Amount	50 uL
Host	Rabbit
Item no.	PRS-15-331-50uL
eClass 6.1	32160702
eClass 9.0	32160702
Available
Alias	NP, MGC117396, MGC125915, MGC125916, PNP, PRO1837, PUNP, NP
Shipping	blue ice or RT
By Research Area	Neuroscience
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-289 of human PNP (NP_000261.2).
Applications	WB: 1:500 - 1:2000 IF: 1:50 - 1:200
Positive Control 1	NCI-H460
Positive Control 2	Mouse liver
Predicted Molecular Weight	Observed: 32kDa
Purification	Affinity purification
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions	Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	PNP
Ncbi Gene Id #	4860
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	PNP
Ncbi Official Full Name	purine nucleoside phosphorylase
Ncbi Organism	Homo sapiens
Swissprot #	P00491
Background	This gene encodes an enzyme which reversibly catalyzes the phosphorolysis of purine nucleosides. The enzyme is trimeric, containing three identical subunits. Mutations which result in nucleoside phosphorylase deficiency result in defective T-cell (cell-mediated) immunity but can also affect B-cell immunity and antibody responses. Neurologic disorders may also be apparent in patients with immune defects. A known polymorphism at aa position 51 that does not affect enzyme activity has been described. A pseudogene has been identified on chromosome 2.
1st Image Caption	Western blot analysis of extracts of various cell lines, using PNP antibody (15-331) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
2nd Image Caption	Immunofluorescence analysis of U2OS cells using PNP antibody (15-331). Blue: DAPI for nuclear staining.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Delivery expected until 8/2/2024

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