Item no. |
PRS-15-220-50uL |
Manufacturer |
ProSci
|
Amount |
50 uL |
Category |
|
Type |
Antibody Primary |
Format |
Liquid |
Applications |
WB |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
H3.3A, H3F3, histone H3.3, H3 histone, family 3A |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Other |
Immunogen |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-136 of human H3F3A (NP_002098.1). |
Applications |
WB: 1:500 - 1:2000 |
Positive Control 1 |
U-87MG |
Positive Control 2 |
HT-29 |
Positive Control 3 |
NCI-H460 |
Positive Control 4 |
HeLa |
Positive Control 5 |
Mouse stomach |
Positive Control 6 |
Mouse thymus |
Predicted Molecular Weight |
Observed: 16kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
H3F3A |
Ncbi Gene Id # |
3020 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
H3F3A |
Ncbi Official Full Name |
H3 histone family member 3A |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P84243 |
Background |
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family. |
1st Image Caption |
Western blot analysis of extracts of various cell lines, using Histone H3.3 antibody (15-220) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.