Comparison

Histone H3.3 Antibody

Item no. PRS-15-220-50uL
Manufacturer ProSci
Amount 50 uL
Category
Type Antibody Primary
Format Liquid
Applications WB
Specific against Human, Mouse, Rat
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias H3.3A, H3F3, histone H3.3, H3 histone, family 3A
Available
Shipping
blue ice or RT
By Research Area
Other
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-136 of human H3F3A (NP_002098.1).
Applications
WB: 1:500 - 1:2000
Positive Control 1
U-87MG
Positive Control 2
HT-29
Positive Control 3
NCI-H460
Positive Control 4
HeLa
Positive Control 5
Mouse stomach
Positive Control 6
Mouse thymus
Predicted Molecular Weight
Observed: 16kDa
Purification
Affinity purification
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions
Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications
None
Ncbi Official Symbol
H3F3A
Ncbi Gene Id #
3020
User Note
Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol
H3F3A
Ncbi Official Full Name
H3 histone family member 3A
Ncbi Organism
Homo sapiens
Swissprot #
P84243
Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family.
1st Image Caption
Western blot analysis of extracts of various cell lines, using Histone H3.3 antibody (15-220) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 50 uL
Available: In stock
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