Manufacturer |
ProSci
|
Category |
|
Type |
Antibody Primary |
Specific against |
Human, Mouse |
Format |
Liquid |
Applications |
WB, IHC |
Amount |
50 uL |
Host |
Rabbit |
Item no. |
PRS-15-076-50uL |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Alias |
Acidic fibroblast growth factor intracellular-binding protein, aFGF intracellular-binding protein, FGF-1 intracellular-binding protein, FIBP |
Shipping |
blue ice or RT |
By Research Area |
Cell Cycle, Growth Factors, Signal Transduction |
Immunogen |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-364 of human FIBP (NP_004205.2). |
Applications |
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200 |
Positive Control 1 |
BxPC-3 |
Positive Control 2 |
Mouse skeletal muscle |
Predicted Molecular Weight |
Observed: 42kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
FIBP |
Ncbi Gene Id # |
9158 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
FIBP |
Ncbi Official Full Name |
Acidic fibroblast growth factor intracellular-binding protein |
Ncbi Organism |
Homo sapiens |
Swissprot # |
O43427 |
Background |
Acidic fibroblast growth factor is mitogenic for a variety of different cell types and acts by stimulating mitogenesis or inducing morphological changes and differentiation. The FIBP protein is an intracellular protein that binds selectively to acidic fibroblast growth factor (aFGF). It is postulated that FIBP may be involved in the mitogenic action of aFGF. Two transcript variants encoding different isoforms have been found for this gene. |
1st Image Caption |
Western blot analysis of extracts of various cell lines, using FIBP antibody (15-076) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s. |
2nd Image Caption |
Immunohistochemistry of paraffin-embedded mouse heart using FIBP antibody (15-076) at dilution of 1:100 (40x lens). |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.