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COPS5 Antibody

COPS5 Antibody

Item no.	PRS-14-971-50uL
Manufacturer	ProSci
Amount	50 uL
Category	Antibodies Immunohistochemistry Western Blot Immunofluorescence Primary Antibodies
Type	Antibody Primary
Format	Liquid
Applications	WB, IF, IHC
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	COPS5, CSN5, JAB1, SGN5, MOV-34
Available
Shipping	blue ice or RT
By Research Area	Cancer, Cell Cycle
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-334 of human COPS5 (NP_006828.2).
Applications	WB: 1:500 - 1:2000 IHC: 1:50 - 1:100 IF: 1:50 - 1:100
Positive Control 1	NIH/3T3
Positive Control 2	MCF7
Positive Control 3	PC-12
Positive Control 4	HeLa
Positive Control 5	K-562
Positive Control 6	DU145
Predicted Molecular Weight	Observed: 37kDa
Purification	Affinity purification
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions	Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	COPS5
Ncbi Gene Id #	10987
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	COPS5
Ncbi Official Full Name	COP9 signalosome subunit 5
Ncbi Organism	Homo sapiens
Swissprot #	Q92905
Background	The protein encoded by this gene is one of the eight subunits of COP9 signalosome, a highly conserved protein complex that functions as an important regulator in multiple signaling pathways. The structure and function of COP9 signalosome is similar to that of the 19S regulatory particle of 26S proteasome. COP9 signalosome has been shown to interact with SCF-type E3 ubiquitin ligases and act as a positive regulator of E3 ubiquitin ligases. This protein is reported to be involved in the degradation of cyclin-dependent kinase inhibitor CDKN1B/p27Kip1. It is also known to be an coactivator that increases the specificity of JUN/AP1 transcription factors.
1st Image Caption	Western blot analysis of extracts of various cell lines, using COPS5 antibody (14-971) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
2nd Image Caption	Immunofluorescence analysis of HeLa cells using COPS5 antibody (14-971). Blue: DAPI for nuclear staining.
3rd Image Caption	Immunofluorescence analysis of C6 cells using COPS5 antibody (14-971) at dilution of 1:100. Blue: DAPI for nuclear staining.
4th Image Caption	Immunofluorescence analysis of NIH/3T3 cells using COPS5 antibody (14-971) at dilution of 1:100. Blue: DAPI for nuclear staining.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 50 uL

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Delivery expected until 9/6/2024

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